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pet21-d(+) vector  (Merck & Co)

 
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    Merck & Co pet21-d(+) vector
    Pet21 D(+) Vector, supplied by Merck & Co, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pet21-d(+)+vector/pet21/10__1016_slash_j__molcatb__2015__10__006-39-9-11
    Average 90 stars, based on 1 article reviews
    pet21-d(+) vector - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Plasmid Preparation:

    Article Title: Enzymatic carboxylation of hydroxystilbenes by the γ-resorcylic acid decarboxylase from Rhizobium radiobacter WU-0108 under reverse reaction conditions
    Article Snippet: Briefly, Escherichia coli BL21(DE3) (Merck, Darmtadt, Germany) with the pET21-d(+) vector (Merck) harboring he gene encoding the decarboxylase fused with a C-terminal exahistidine-tag was cultivated for 16 h at 30 ◦C in 100 mL of uria-Bertani medium containing 50 g/mL of carbenicillin and .1 mM isopropylthiogalactopyranoside, with shaking at 180 rpm. he enzyme was purified from the cell lysate by Ni2+ affinity hromatography using a His GraviTrap column (GE Healthcare, ittle Chalfont, UK) and desalted by gel permeation chromatograhy using a PD-10 column (GE Healthcare).

    Purification:

    Article Title: Enzymatic carboxylation of hydroxystilbenes by the γ-resorcylic acid decarboxylase from Rhizobium radiobacter WU-0108 under reverse reaction conditions
    Article Snippet: Briefly, Escherichia coli BL21(DE3) (Merck, Darmtadt, Germany) with the pET21-d(+) vector (Merck) harboring he gene encoding the decarboxylase fused with a C-terminal exahistidine-tag was cultivated for 16 h at 30 ◦C in 100 mL of uria-Bertani medium containing 50 g/mL of carbenicillin and .1 mM isopropylthiogalactopyranoside, with shaking at 180 rpm. he enzyme was purified from the cell lysate by Ni2+ affinity hromatography using a His GraviTrap column (GE Healthcare, ittle Chalfont, UK) and desalted by gel permeation chromatograhy using a PD-10 column (GE Healthcare).



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